Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-76082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Suppression of NLRP3 Inflammasome, Pyroptosis, and Cell Death by NIM811 in Rotenone-Exposed Cells as an in vitro Model of Parkinson's Disease.
Zhang M, He Q, Chen G, Li PA
Neuro-degenerative diseases 2020;20(2-3):73-83
Neuro-degenerative diseases 2020;20(2-3):73-83
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-18. Samples were incubated with IL-18 polyclonal antibody (Product # PA5-76082).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IL-18 Polyclonal Antibody (Product # PA5-76082) and a 22kDa band corresponding to Interleukin-18 was observed across cell lines tested except HEK-293 and Raji which is reported to be low. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), HEK-293 (Lane 3) and Raji (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6. Influences of rotenone and NIM811 on caspase-1-dependent pyroptotic pathways. a-e Western blots showing relative intensity of NLRP3, pro-caspase-1, caspase-1, GSDMD, and IL-18 protein expression. f IL-1beta activity assessed by ELISA. Data were collected from 3 independent experiments and presented as means +- SD of 3 independent experiments. * p < 0.05, ** p < 0.01 versus VC group; # p < 0.05 versus rotenone 4.0-muM group.