A00124-1

antibody from Boster Biological Technology

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00124-1

Provider

Boster Biological Technology

Product name

Anti-IL18 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for IL18 detection. Tested with WB, FCM, Direct ELISA in Mouse.

Reactivity

Mouse

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of Ana-1 cells using anti-IL18 antibody (A00124-1). Overlay histogram showing Ana-1 cells stained with A00124-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL18 Antibody (A00124-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image