RP1017

antibody from Boster Biological Technology

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RP1017

Provider

Boster Biological Technology

Product name

Anti-Interleukin-18 IL18 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for IL 1F4/IL18 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. IL 1F4/IL18 information: Molecular Weight: 22135 MW; Subcellular Localization: Secreted.

Reactivity

Mouse

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Betaine eliminates CFA-induced depressive-like behaviour in mice may be through inhibition of microglia and astrocyte activation and polarization.

Liang Y, Chen L, Huang Y, Xie L, Liu X, Zhou W, Cao W, Chen Z, Zhong X
Brain research bulletin 2024 Jan;206:110863

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Hydroxysafflor yellow A mitigates myocardial fibrosis induced by isoproterenol and angiotensin II.

Cong P, Huang G, Zhao Y, Lan Y
American journal of translational research 2022;14(12):8588-8598

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Targeting PERK mediated endoplasmic reticulum stress attenuates neuroinflammation and alleviates lipopolysaccharide-induced depressive-like behavior in male mice.

Xu XF, Shi MM, Luo MY, Liu DD, Guo DM, Ling C, Zhong XL, Xu Y, Cao WY
International immunopharmacology 2022 Oct;111:109092

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Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis.

Chen L, Liu D, Mao M, Liu W, Wang Y, Liang Y, Cao W, Zhong X
Molecular nutrition & food research 2022 Nov;66(22):e2200341

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Fibrinogen-like protein 2 aggravates nonalcoholic steatohepatitis via interaction with TLR4, eliciting inflammation in macrophages and inducing hepatic lipid metabolism disorder.

Hu J, Wang H, Li X, Liu Y, Mi Y, Kong H, Xi D, Yan W, Luo X, Ning Q, Wang X
Theranostics 2020;10(21):9702-9720

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Carnosic acid alleviates brain injury through NF‑κB‑regulated inflammation and Caspase‑3‑associated apoptosis in high fat‑induced mouse models.

Liu Y, Zhang Y, Hu M, Li YH, Cao XH
Molecular medicine reports 2019 Jul;20(1):495-504

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Role of interleukin 18 in acute lung inflammation induced by gut ischemia reperfusion.

Yang YJ, Shen Y, Chen SH, Ge XR
World journal of gastroenterology 2005 Aug 7;11(29):4524-9

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of IL-18 using anti- IL-18 antibody (RP1017). IL-18 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- IL-18 Antibody (RP1017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image