MA5-55867

antibody from Invitrogen Antibodies

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-55867

Provider

Invitrogen Antibodies

Product name

IL-18 Monoclonal Antibody (K94016_16G4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

K94016_16G4

Vial size

50 μg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of IL-18 in 200 µg of U-2 OS lysate. Samples are as follows: Lane 1: U-2 OS lysate, Lane 2: IL-18 immunoprecipitated from U-2 OS lysate, Lane3: The same as Lane 2 but KT82 was used as IgG isotype control antibody. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE, blotted onto nitrocellulose membrane, and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. The isotype control antibody was KT82. Incubation of samples with IL-18 monoclonal antibody (Product # MA5-55867) at a dilution of 2.5 µg was used.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IL-18 in paraffin-embedded tonsil tissue. Sample was incubated with IL-18 monoclonal antibody (Product # MA5-55867) at a dilution of 2 µg/mL (RT, 1 hour). Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.