TA504194

antibody from OriGene

Targeting: JCHAIN IGCJ, IGJ, JCH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504194

Provider

OriGene

Proper citation

OriGene Cat#TA504194, RRID:AB_11126558

Product name

IGJ mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Antibody type

Monoclonal

Description

IGJ mouse monoclonal antibody, clone OTI3H7 (formerly 3H7)

Host

Mouse

Conjugate

Unconjugated

Epitope

JCHAIN

Isotype

IgG

Antibody clone number

OTI3H7

Vial size

100 µl

Concentration

0.43 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGJ (RC207932, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGJ.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-IGJ monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IGJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504194)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IGJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504194)

Validation comment

IHC