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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-31311 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RIPX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, mouse brain. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (88%), Xenopus laevis (94%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Neuronally Enriched RUFY3 Is Required for Caspase-Mediated Axon Degeneration.
Hertz NT, Adams EL, Weber RA, Shen RJ, O'Rourke MK, Simon DJ, Zebroski H, Olsen O, Morgan CW, Mileur TR, Hitchcock AM, Sinnott Armstrong NA, Wainberg M, Bassik MC, Molina H, Wells JA, Tessier-Lavigne M
Neuron 2019 Aug 7;103(3):412-422.e4
Neuron 2019 Aug 7;103(3):412-422.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RIPX Polyclonal Antibody (Product # PA5-31311). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. RIPX Polyclonal Antibody (Product # PA5-31311) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RIPX Polyclonal Antibody (Product # PA5-31311). Sample (30 µg of whole cell lysate). Lane A: IMR32 . 7.5% SDS PAGE. RIPX Polyclonal Antibody (Product # PA5-31311) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U251 xenograft, using RIPX (Product # PA5-31311) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RIPX Polyclonal Antibody detects RIPX protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. RIPX Polyclonal Antibody (Product # PA5-31311) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
