RQ5346

antibody from NSJ Bioreagents

Targeting: HAPLN1 CRTL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5346

Provider

NSJ Bioreagents

Product name

FLOT1 Antibody / Flotillin 1

Antibody type

Monoclonal

Description

This highly specific FLOT1 antibody is suitable for use in Immunohistochemistry/Western blot applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

BHB-8

Vial size

100 ul

Concentration

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Storage

Store the FLOT1 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) K562 cell lysate with FLOT1 antibody. Predicted molecular weight ~49 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human U-2 OS 2) human 293T, 3) human A431, 4) human PC-3, 5) rat brain, 6) rat lung, 7) mouse brain and 8) mouse lung tissue lysate with FLOT1 antibody. Predicted molecular weight ~49 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human uterus with FLOT1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung squamous cell carcinoma tissue with FLOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with FLOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer tissue with FLOT1 antibody. HIER: boil tissue sections in pH8 EDTA for 10-20 min and allow to cool before testing.