RQ5347

antibody from NSJ Bioreagents

Targeting: HAPLN1 CRTL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5347

Provider

NSJ Bioreagents

Product name

HAPLN1 Antibody

Antibody type

Monoclonal

Description

This highly specific HAPLN1 antibody is suitable for use in Immunohistochemistry/Western blot/Immunofluorescence applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antibody clone number

BHA-8

Vial size

100 ul

Concentration

Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

Storage

Store the HAPLN1 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Caco-2 and 2) mouse spleen lysate with HAPLN1 antibody. Expected molecular weight: 41-48 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Caco-2 and 2) mouse spleen lysate with HAPLN1 antibody. Expected molecular weight: 41-48 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) Jurkat, 3) MCF7 and 4) SH-SY5Y cell lysate with HAPLN1 antibody at 1:1000. Expected molecular weight: 41-48 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) A549, 2) NCI-H1299 and 3) HUVEC lysate with HAPLN1 antibody at 1:1000. Expected molecular weight: 41-48 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse liver, 2) mouse spleen, 3) mouse brain, 4) rat heart, 5) rat liver, 6) rat kidney and 7) rat brain tissue lysate with HAPLN1 antibody at 1:1000. Expected molecular weight: 41-48 kDa depending on glycosylation level.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human HeLa cells with HAPLN1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human spleen tissue with HAPLN1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human glioblastoma tissue with HAPLN1 antibody at 1:50. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse heart tissue with HAPLN1 antibody at 1:50. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse skeletal muscle tissue with HAPLN1 antibody at 1:50. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with HAPLN1 antibody at 1:50. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.