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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN954321 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1) (N-Term), (AA 17-46) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of Human Proteoglycan link protein / HAPLN1
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term,AA 17-46
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Glypican 5 is an interferon-beta response gene: a replication study.
Protumorigenic role of HAPLN1 and its IgV domain in malignant pleural mesothelioma.
Cénit MD, Blanco-Kelly F, de las Heras V, Bartolomé M, de la Concha EG, Urcelay E, Arroyo R, Martínez A
Multiple sclerosis (Houndmills, Basingstoke, England) 2009 Aug;15(8):913-7
Multiple sclerosis (Houndmills, Basingstoke, England) 2009 Aug;15(8):913-7
Protumorigenic role of HAPLN1 and its IgV domain in malignant pleural mesothelioma.
Ivanova AV, Goparaju CM, Ivanov SV, Nonaka D, Cruz C, Beck A, Lonardo F, Wali A, Pass HI
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2602-11
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Apr 15;15(8):2602-11
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of HAPLN1 Antibody (N-term) Cat.-No AP51999PU-N in MCF-7,Jurkat,HepG2 cell line lysates (35ug/lane). This demonstrates the HAPLN1 antibody detected the HAPLN1 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded prostate carcinoma reacted with HAPLN1 Antibody (N-term) Cat.-No AP51999PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MCF-7 cells using HAPLN1 Antibody (N-term) Cat.-No AP51999PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
