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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Protein array [1]
- Other assay [2]
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- Product number
- V7947 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant MCM7 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant MCM7 antibody is suitable for use in Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- rMCM7/1468
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples with recombinant MCM7 antibody (clone rMCM7/1468). Expected molecular weight: 80-90 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples with recombinant MCM7 antibody (clone rMCM7/1468). Expected molecular weight: 80-90 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A431 cell lysate with recombinant MCM7 antibody (clone rMCM7/1468). Expected molecular weight: 80-90 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with recombinant MCM7 antibody (clone rMCM7/1468). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil with recombinant MCM7 antibody (clone rMCM7/1468). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Protein array validation of the MCM7 antibody: Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant MCM7 antibody (clone rMCM7/1468). These results demonstrate the foremost specificity of the rMCM7/1468 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant MCM7 antibody (clone rMCM7/1468) as confirmation of integrity and purity.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant MCM7 antibody (clone rMCM7/1468) as confirmation of integrity and purity.
