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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunohistochemistry [1]
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- Product number
- NB100-56176 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56176, RRID:AB_839028
- Product name
- Rabbit Polyclonal TRAF-3 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. TRAF3
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 1;165(9):5084-96
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.
