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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- V2908 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GRP94 Antibody / HSP90B1
- Antibody type
- Monoclonal
- Description
- This highly specific GRP94 antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- 9G10.F8.2
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the GRP94 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysate with GRP94 antibody (clone 9G10.F8.2). Expected molecular weight: 94~96kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysate with GRP94 antibody (clone 9G10.F8.2). Expected molecular weight: 94~96kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HepG2 cells with GRP94 antibody (clone 9G10.F8.2, green) and Reddot nuclear stain (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human breast carcinoma stained with GRP94 antibody (9G10.F8.2).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with GRP94 antibody (clone 9G10.F8.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with GRP94 antibody (clone 9G10.F8.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry staining of PFA-fixed human HepG2 cells with GRP94 antibody; Red=isotype control, Blue= GRP94 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free GRP94 antibody (clone 9G10.F8.2) as confirmation of integrity and purity.
