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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- AM09037PU-N - Provider product page
- Provider
- OriGene
- Product name
- GRP94 (HSP90B1) (676-803) mouse monoclonal antibody, clone 2H3, Purified
- Antibody type
- Monoclonal
- Description
- GRP94 (HSP90B1) (676-803) mouse monoclonal antibody, clone 2H3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSP90B1
- Isotype
- IgG
- Antibody clone number
- 2H3
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
Submitted references TAS-116, a highly selective inhibitor of heat shock protein 90α and β, demonstrates potent antitumor activity and minimal ocular toxicity in preclinical models.
Ohkubo S, Kodama Y, Muraoka H, Hitotsumachi H, Yoshimura C, Kitade M, Hashimoto A, Ito K, Gomori A, Takahashi K, Shibata Y, Kanoh A, Yonekura K
Molecular cancer therapeutics 2015 Jan;14(1):14-22
Molecular cancer therapeutics 2015 Jan;14(1):14-22
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: The cell lysates of hES (20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with HSP90B1 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 ug/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
- Validation comment
- IHC
