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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB1085 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1085, RRID:AB_1577825
- Product name
- HSP90B1 monoclonal antibody, clone 2H3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant HSP90B1.
- Isotype
- IgG
- Antibody clone number
- 2H3
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan.
TRA-1-60: a new serum marker in patients with germ-cell tumors.
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Badcock G, Pigott C, Goepel J, Andrews PW
Cancer research 1999 Sep 15;59(18):4715-9
Cancer research 1999 Sep 15;59(18):4715-9
TRA-1-60: a new serum marker in patients with germ-cell tumors.
Marrink J, Andrews PW, van Brummen PJ, de Jong HJ, Sleijfer DT, Schraffordt Koops H, Oosterhuis JW
International journal of cancer 1991 Sep 30;49(3):368-72
International journal of cancer 1991 Sep 30;49(3):368-72
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P
Hybridoma 1984 Winter;3(4):347-61
Hybridoma 1984 Winter;3(4):347-61
Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells.
Andrews PW, Banting G, Damjanov I, Arnaud D, Avner P
Hybridoma 1984 Winter;3(4):347-61
Hybridoma 1984 Winter;3(4):347-61
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human ES cell lysate (20 ug) was resolved by SDS - PAGE , transferred to PVDF membrane and probed with HSP90B1 monoclonal antibody , clone 2H3 (1 : 500). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human ES cell colony with HSP90B1 monoclonal antibody , clone 2H3 (Cat # MAB1085) .
- Validation comment
- Immunocytochemistry
