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- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- NB300-619 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-619, RRID:AB_2295607
- Product name
- Rat Monoclonal gp96/HSP90B1/GRP94 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. GRP94 (9G10)
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Hamster
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100uL
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion.
Vierra NC, Dickerson MT, Jordan KL, Dadi PK, Katdare KA, Altman MK, Milian SC, Jacobson DA
Molecular metabolism 2018 Mar;9:84-97
Molecular metabolism 2018 Mar;9:84-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse liver tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Analysis of Glucose-Regulated Protein 94 (Grp94) in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (from Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor, probed with a Grp94 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-rat IgG (H+L) secondary antibody at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Staining on poorly differentiated adenocarcinoma from paraffin-embedded human colon tissue sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (9G10) [NB300-619] - Normal biopsies of deparaffinized Mouse breast tissue.
