Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [8]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-81803 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-81803, RRID:AB_11013701
- Product name
- Rabbit Polyclonal gp96/HSP90B1/GRP94 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human gp96/HSP90B1/GRP94 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteomic analyses reveal high expression of decorin and endoplasmin (HSP90B1) are associated with breast cancer metastasis and decreased survival.
Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis.
Cawthorn TR, Moreno JC, Dharsee M, Tran-Thanh D, Ackloo S, Zhu PH, Sardana G, Chen J, Kupchak P, Jacks LM, Miller NA, Youngson BJ, Iakovlev V, Guidos CJ, Vallis KA, Evans KR, McCready D, Leong WL, Done SJ
PloS one 2012;7(2):e30992
PloS one 2012;7(2):e30992
Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis.
Ito A, Mimae T, Yamamoto YS, Hagiyama M, Nakanishi J, Ito M, Hosokawa Y, Okada M, Murakami Y, Kondo T
Laboratory investigation; a journal of technical methods and pathology 2012 Sep;92(9):1374-85
Laboratory investigation; a journal of technical methods and pathology 2012 Sep;92(9):1374-85
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human cell line SK-BR-3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis using Anti-HSP90B1 antibody NBP1-81803 (A) shows similar pattern to independent antibody NBP1-81802 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.