NBP1-81803

antibody from Novus Biologicals

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-81803

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-81803, RRID:AB_11013701

Product name

Rabbit Polyclonal gp96/HSP90B1/GRP94 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human gp96/HSP90B1/GRP94 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Proteomic analyses reveal high expression of decorin and endoplasmin (HSP90B1) are associated with breast cancer metastasis and decreased survival.

Cawthorn TR, Moreno JC, Dharsee M, Tran-Thanh D, Ackloo S, Zhu PH, Sardana G, Chen J, Kupchak P, Jacks LM, Miller NA, Youngson BJ, Iakovlev V, Guidos CJ, Vallis KA, Evans KR, McCready D, Leong WL, Done SJ
PloS one 2012;7(2):e30992

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Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis.

Ito A, Mimae T, Yamamoto YS, Hagiyama M, Nakanishi J, Ito M, Hosokawa Y, Okada M, Murakami Y, Kondo T
Laboratory investigation; a journal of technical methods and pathology 2012 Sep;92(9):1374-85

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human cell line SK-BR-3.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis using Anti-HSP90B1 antibody NBP1-81803 (A) shows similar pattern to independent antibody NBP1-81802 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Analysis in human placenta and skeletal muscle tissues. Corresponding gp96/HSP90B1/GRP94 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody [NBP1-81803] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.