F54810

antibody from NSJ Bioreagents

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54810

Provider

NSJ Bioreagents

Product name

HSP90B1 Antibody / GRP94

Antibody type

Polyclonal

Description

This highly specific HSP90B1 antibody is suitable for use in Immunofluorescence/Flow cytometry/Immunohistochemistry/Western blot applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the HSP90B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human HepG2, 2) mouse liver, 3) hamster CHO, 4) human K562 and 5) human HeLa cell lysate with HSP90B1 antibody. Predicted molecular weight ~94 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human NCI-H460 cells with HSP90B1 antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung carcinoma tissue with HSP90B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with HSP90B1 antibody; Blue=isotype control, Green= HSP90B1 antibody.