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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-27860 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRP94 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, NIH-3T3, endothelial cells.
- Concentration
- 0.95 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GRP94 Polyclonal Antibody (Product # PA5-27860). Sample (30 µg of whole cell lysate). A: H1299. B: Hela S3. 7.5% SDS PAGE. GRP94 Polyclonal Antibody (Product # PA5-27860) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GRP94 Polyclonal Antibody (Product # PA5-27860). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3 . 7.5% SDS PAGE. GRP94 Polyclonal Antibody (Product # PA5-27860) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GRP94 Polyclonal Antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GRP94 protein stained by GRP94 Polyclonal Antibody (Product # PA5-27860) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B (Product # PA5-27860) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GRP94 was performed in HeLa whole cell extracts using 5 µg of GRP94 Polyclonal Antibody (Product # PA5-27860). Samples were transferred to a membrane and probed with GRP94 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
