PA5-27866

antibody from Invitrogen Antibodies

Targeting: HSP90B1 GP96, GRP94, TRA1

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-27866 - Provider product page
Provider: Invitrogen Antibodies
Product name: GRP94 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, NIH3T3, PC-12, Rat-2. Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (83%), Xenopus laevis (84%), Dog (95%), Pig (95%), Chicken (91%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

HSP90B1 protein structure - PA5-27866 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GRP94/HSP90B1 using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GRP94/HSP90B1 polyclonal antibody (Product # PA5-27866) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GRP94/HSP90B1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GRP94/HSP90B1 polyclonal antibody (Product # PA5-27866) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of GRP94/HSP90B1 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GRP94/HSP90B1 polyclonal antibody (Product # PA5-27866) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRP94 was performed by separating 30 µg of whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GRP94 Polyclonal Antibody (Product # PA5-27866) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRP94 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a GRP94 Polyclonal Antibody (Product # PA5-27866) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of GRP94 was performed by separating 30 µg of various whole cell extracts by 7.5 % SDS-PAGE. Proteins were transferred to a membrane and probed with a GRP94 Polyclonal Antibody (Product # PA5-27866) at a dilution of 1:1,000.