AP52118PU-N

antibody from Acris Antibodies GmbH

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP52118PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP52118PU-N, RRID:AB_11148839

Product name

anti Endoplasmin / HSP90B1 / TRA1 (Center)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 467-495 amino acids from the Central region of human HSP90B1

Reactivity

Human

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Endoplasmin / HSP90B1 / TRA1 Antibody (Center) Cat.-No AP52118PU-N in K562, A549, Hela, NCI-H460, NCI-H292, ZR-75-1 cell line lysates (35ug/lane). This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with Endoplasmin / HSP90B1 / TRA1 Antibody (Center) Cat.-No AP52118PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of A549 cells using Endoplasmin / HSP90B1 / TRA1 Antibody (Center) Cat.-No AP52118PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis