ABIN1504500

antibody from antibodies-online

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN1504500

Provider

antibodies-online

Product name

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Rat, Simian

Host

Rabbit

Isotype

IgG

Vial size

0.1 mL

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot Analysis (Representative lot data). Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-GRP94 (1:500 dilution of this lot). Proteins were visualized using a donkey antirabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein GRP94 (~94 kDa).

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western Blot Analysis (Representative lot data): Lysates from Jurkat (Lane1), HEK293 (Lane 2), HUVEC(Lane 3), PC12 (Lane 4), A431 (Lane 5), L6 (Lane 6), HepG2 (Lane 7), HeLa (Lane 8) and PC3 (Lane 9) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Glucose Regulated Protein 94 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti rabbitsecondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Glucose Regulated Protein 94 (~94 kDa).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunocytochemistry (Representative image from a previous lot): Rabbit anti-GRP94 localization of GRP94 (green) in HEK293 cells. Blue staining is DAPI which stains the nucleus of the cell. Previous lots of this antibody showed positive testing for immunocytochemistry on HeLa, COS7 and HEK293 cells