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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AP7087b - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7087b, RRID:AB_2277098
- Product name
- DCK Antibody (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME
Blood 2009 Mar 5;113(10):2145-53
Blood 2009 Mar 5;113(10):2145-53
Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Lamba JK, Crews K, Pounds S, Schuetz EG, Gresham J, Gandhi V, Plunkett W, Rubnitz J, Ribeiro R
The Journal of pharmacology and experimental therapeutics 2007 Dec;323(3):935-45
The Journal of pharmacology and experimental therapeutics 2007 Dec;323(3):935-45
No comments: Submit comment
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- The anti-DCK Pab (Cat. #AP7087b) is used in Western blot to detect DCK in mouse intestine tissue lysate.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of DCK Antibody (C-term)(Cat#AP7087b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Deoxycytidine kinase(DCK) immunoprecipitated from H460cells with 7.5ug (microgram) of the dCK antibody (AP7087b) using the Pierce classic mammalian IP kit (#45217) reagent as described as manufacturer instructions (lane 1, 3) and Current Protocols in Cell Biology, 1998, 7.2.1-7.2.21. Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1:700 dilution of DCK antibody (AP7087b). Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit). No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago."
- Primary Ab dilution
- 1:500~1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- DCK Antibody (C-term) (Cat. #AP7087B)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DCK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
