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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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- Product number
- GTX632065 - Provider product page
- Provider
- GeneTex
- Product name
- DCK antibody [GT10412]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX632065) diluted at a dilution of 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis.Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DCK protein from HCT116 whole cell extracts using 5 £gg of DCK antibody [GT10412] (GTX632065), DCK antibody (GTX107636) or DCK antibody (GTX102800).Western blot analysis was performed using DCK antibody [GT10412] (GTX632065).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 ?g of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800).Western blot analysis was performed using DCK antibody [GT10412] (GTX632065).EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
