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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- PAB2020 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2020, RRID:AB_1574251
- Product name
- DCK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DCK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Population-specific genetic variants important in susceptibility to cytarabine arabinoside cytotoxicity.
Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Hartford CM, Duan S, Delaney SM, Mi S, Kistner EO, Lamba JK, Huang RS, Dolan ME
Blood 2009 Mar 5;113(10):2145-53
Blood 2009 Mar 5;113(10):2145-53
Pharmacogenetics of deoxycytidine kinase: identification and characterization of novel genetic variants.
Lamba JK, Crews K, Pounds S, Schuetz EG, Gresham J, Gandhi V, Plunkett W, Rubnitz J, Ribeiro R
The Journal of pharmacology and experimental therapeutics 2007 Dec;323(3):935-45
The Journal of pharmacology and experimental therapeutics 2007 Dec;323(3):935-45
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The DCK polyclonal antibody (Cat # PAB2020) is used in Western blot to detect DCK in mouse intestine tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Deoxycytidine kinase (DCK) immunoprecipitated from NCI-H460 cells with 7.5 ug (microgram) of DCK polyclonal antibody (Cat # PAB2020) using the Pierce classic mammalian IP kit reagent as described as manufacturer instructions (lane 1, 3) and current protocols in Cell Biology, 1998, 7.2.1-7.2.21.Proteins separated on a 12% SDS gel, transferred to a PVDF membrane and probed with 1 : 700 dilution of DCK polyclonal antibody (Cat # PAB2020).Bands were detected using enhanced chemiluminescence (SuperSignal West Pico Chemiluminescent Substrate Kit).No specific reagents were employed to remove IgG from immunoprecipitated sample. Data courtesy of Dr. Stacy Shord, University of Illinois, Chicago.
- Validation comment
- Immunoprecipitation
