MA5-27740

antibody from Invitrogen Antibodies

Targeting: DCK

Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-27740 - Provider product page
Provider: Invitrogen Antibodies
Product name: DCK Monoclonal Antibody (GT10412)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Positive Control: A431, HeLa, HepG2, 293T, A549, H1299, HCT116, Neuro 2A, C8D30, NIH-3T3, Raw264.7, PC-12, Rat2 Predicted Reactivity: Rhesus Monkey (96%), Bovine (95%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: GT10412
Vial size: 100 µL
Concentration: 2.01 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

DCK protein structure - MA5-27740 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCK in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with DCK monoclonal antibody (Product # MA5-27740) using a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCK in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with DCK monoclonal antibody (Product # MA5-27740) using a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCK in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with DCK monoclonal antibody (Product # MA5-27740) using a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCK in whole cell lysate using 30 µg of protein. Samples were separated with 12% SDS-PAGE and incubated with DCK monoclonal antibody (Product # MA5-27740) using a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Polyclonal Antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK Polyclonal Antibody DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) diluted at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of DCK was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) at a dilution of 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using DCK Monoclonal Antibody (GT10412) (Product # MA5-27740). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Polyclonal Antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK Polyclonal Antibody DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) diluted at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Monoclonal Antibody (GT10412) detects DCK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) diluted at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Monoclonal Antibody (GT10412) detects DCK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK Monoclonal Antibody (GT10412) (Product # MA5-27740) diluted at a dilution of 1:500.