PA5-21846

antibody from Invitrogen Antibodies

Targeting: DCK

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-21846 - Provider product page
Provider: Invitrogen Antibodies
Product name: DCK Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, A549, mouse brain, PC-12, Rat2. Predicted reactivity: Mouse (93%), Rat (92%), Chicken (82%), Rhesus Monkey (96%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

DCK protein structure - PA5-21846 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Deoxycytidine kinase using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Deoxycytidine kinase polyclonal antibody (Product # PA5-21846) at a dilution of 1:5000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of DCK was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DCK Polyclonal Antibody (Product # PA5-21846) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of DCK was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DCK Polyclonal Antibody (Product # PA5-21846) at a dilution of 1:5000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Polyclonal Antibody detects DCK protein by western blot analysis. A. 30 µg PC-12 whole cell extract. B. 30 µg Rat2 whole cell extract.12% SDS-PAGE. DCK Polyclonal Antibody (Product # PA5-21846) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of DCK was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DCK Polyclonal Antibody (Product # PA5-21846) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DCK Polyclonal Antibody detects DCK protein by western blot analysis. A. 50 µg mouse brain extract.12% SDS-PAGE. DCK Polyclonal Antibody (Product # PA5-21846) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.