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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-72848 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYH3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibodies should not be exposed to prolonged high temperatures.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 Polyclonal Antibody (Product # PA5-72848) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MYH3 Polyclonal Antibody (Product # PA5-72848) and a 230 kDa band corresponding to MYH3 was observed in Rat heart in comparison to Rat testis which is reported to be negative. Tissue extracts (30 µg lysate) of Rat Heart (Lane 1) and Rat Testis (Lane 2) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 Polyclonal Antibody (Product # PA5-72848) at 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 Polyclonal Antibody (Product # PA5-72848) at 5 µg/mL.
