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LearnAb176388
antibody from Aladdin Scientific
Targeting: PGF
D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [3]
- Flow cytometry [2]
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- Product number
- Ab176388 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PLGF Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human PLGF Antibody, Monoclonal (5D9-3), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA.CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AEFLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 5D9-3
- Vial size
- 100_g,10_g,1mg,50_g
- Concentration
- Lot by Lot
- Storage
- Store at 37¡C short term_7 days_Store at 4¡C short term_30 days_. Store at -20±5¡C long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176388) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176386) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Sandwich ELISA - PLGF protein standard curve Human PLGF protein was serially diluted 2-fold and captured by PLGF Mouse mAb (Ab176386) coated on a Clear Polystyrene Microplate (W291037). PLGF Mouse mAb (Ab176388) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Binding ELISA - PLGF Mouse mAb (Ab176388) Immobilized Recombinant Human PLGF Protein at 1.0 μg/mL can bind PLGF Mouse mAb (Ab176388) with the EC₅₀ of 38.54 ng/mL.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176388) ·Detection antibody: PLGF Mouse mAb (Ab176386)
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Relevance of detection results between aladdin Antibody Pair (Ab176386 & Ab176388) in Flow Fluorescence ·Capture antibody: PLGF Mouse mAb (Ab176386) ·Detection antibody: PLGF Mouse mAb (Ab176388)
