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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX114283 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114283, RRID:AB_11177115
- Product name
- BNIP2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE GTX114283 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BNIP2 antibody detects BNIP2 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody (GTX114283) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BNIP2 antibody (GTX114283) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BNIP2 antibody detects BNIP2 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: BNIP2 protein stained by BNIP2 antibody (GTX114283) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U87 xenograft, using BNIP2(GTX114283) antibody at 1:500 dilution.
