- PA5-45744 - Invitrogen Antibodies DNAH9 antibody

Submitted references Dnah9 mutant mice and organoid models recapitulate the clinical features of patients with PCD and provide an excellent platform for drug screening.

Zheng R, Yang W, Wen Y, Xie L, Shi F, Lu D, Luo J, Li Y, Zhang R, Chen T, Chen L, Xu W, Liu H
Cell death & disease 2022 Jun 21;13(6):559

Genetic Defects in DNAH2 Underlie Male Infertility With Multiple Morphological Abnormalities of the Sperm Flagella in Humans and Mice.

Hwang JY, Nawaz S, Choi J, Wang H, Hussain S, Nawaz M, Lopez-Giraldez F, Jeong K, Dong W, Oh JN, Bilguvar K, Mane S, Lee CK, Bystroff C, Lifton RP, Ahmad W, Chung JJ
Frontiers in cell and developmental biology 2021;9:662903

Quantifying Ciliary Dynamics during Assembly Reveals Stepwise Waveform Maturation in Airway Cells.

Oltean A, Schaffer AJ, Bayly PV, Brody SL
American journal of respiratory cell and molecular biology 2018 Oct;59(4):511-522

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of mouse tracheal epithelial cells using an anti-Dynein Heavy chain polyclonal antibody (Product # PA5-45744). Primary Antibody Dilution:1:50; Secondary Antibody: Anti-rabbit alexa 488; Secondary Antibody Dilution: 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 3 Flagellar ultrastructure is disorganized, and axonemal proteins express abnormally in Dnah2 - sperm. (A,B) Scanning electron microscopy images of Dnah2 +/Delta (A) and Dnah2 Delta/Delta (B) sperm. Dnah2 Delta/Delta sperm display absence of ( left ) or an irregular ( right ) mitochondrial helical sheath in the midpiece of the sperm tail, along with abnormal head shapes. Arrowheads indicate annulus. H, head; MP, midpiece; and PP, principal piece. (C,D) Transmission electron microscopy images of Dnah2 +/Delta (C) and Dnah2 Delta/Delta (D) sperm. Representative cross-section images of TEM reveal conformational defects in the midpiece of Dnah2 Delta/Delta sperm. Incomplete 9+2 axonemal structure ( left ), delocalized outer dense fibers (ODF, arrow) and microtubule doublet ( middle , arrowhead), and lack of the axonemal components ( right ) were observed in the Dnah2 Delta/Delta sperm. Inner (yellow asterisk) and outer (white asterisk) dynein arm structure were observed from microtubule doublet occasionally ( left , inset; scale bar = 50 nm). Microtubule doublets are numbered with the absence of the corresponding microtubule doublet in red. M, mitochondria. (E) Confocal fluorescence images of axonemal proteins in WT and Dnah2 Delta/Delta sperm. Dnah2 Delta/Delta sperm do not express another IDA component (DNAH1, magenta) and a radial spoke protein (RSPH3, green) but an ODA protein (DNAH9, yellow). Flagella microtubules (AcTub, white) and sperm heads (Hoechst, blue) ser

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Ciliated cells from Dnah9 KD mice show reduced expression of interactors CCDC114 and GAS8 protein. A Immunostaining analysis of DNAH9, CCDC114, LLRC6, and GAS8 in ciliated cells from Dnah9 KD nasal epithelium. Green, ace-tubulin; red, DNAH9, LRRC6, CCDC114, and GAS8; blue, DAPI; Scale bar = 10 um. B Results of immunofluorescence images quantification corresponding to ciliated cells in ( A ). * P < 0.05; ns no significance, n = 3 per group. C Western blot analysis showing the protein expression of CCDC114, GAS8, and LRRC6 in lung tissues of WT and Dnah9 KD mice. GAPDH is the endogenous control. The right pattern is the statistical analysis of relative protein intensities revealed by western blot. * P < 0.05; ns no significance, n = 3 per group. D Co-IP results demonstrating interactions between DNAH9 and CCDC114/GAS8.

PA5-45744

Antibody data