BS5859
antibody from Bioworld Technology, Inc
Targeting: PKN1
DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- BS5859 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PKN1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 294-339 of Human PKN1.
- Description
- Protein kinase N (PKN) is a fatty acid-activated serine/threonine kinase whose catalytic domain exhibits homology with that of the PKC family. PKN associates with Rho via its amino terminus, is activated in a GTP-dependent manner and phosphorylates the head-rod domain of neurofilament protein. A second protein, rhophilin, exhibits 40% sequence identity with the amino terminal Rho binding domain. The enzymatic activity of rhophilin has not been demonstrated and it is possible that it acts through the recruitment of cytoskeletal components that initiate a kinase signaling cascade. Citron interacts specifically with active Rho and Rac1 but not Cdc42. Citron exhibits a distinctive protein organization and little homology with the Rho binding domains of PKN and rhophilin.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of PKN1 pAb at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:NIH-3T3 cell lysate