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LearnH00005585-M01
antibody from Abnova Corporation
Targeting: PKN1
DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005585-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005585-M01, RRID:AB_464012
- Product name
- PKN1 monoclonal antibody (M01), clone 1B10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant PKN1.
- Antigen sequence
LDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSK
QQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGT
FSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKS
SRDPPSSPSSLSSPIQESTAPELPSETQETPGPAL
CSPLRKSPLTLEDF- Isotype
- IgG
- Antibody clone number
- 1B10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PKN1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PKN1 monoclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
