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LearnH00005585-M01
antibody from Novus Biologicals
Targeting: PKN1
DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00005585-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005585-M01, RRID:AB_547088
- Product name
- Mouse Monoclonal PKN1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PKN1 - protein kinase N1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PKN1 Antibody (1B10) [H00005585-M01] - Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of monoclonal antibody to PKN1 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.
