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LearnMA5-31849
antibody from Invitrogen Antibodies
Targeting: PKN1
DBK, MGC46204, PAK1, PKN, PRK1, PRKCL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-31849 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKN1 Monoclonal Antibody (4H10B1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H10B1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKN1 in human PKN1 recombinant protein. Sample was incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PKN1 in HEK293 (1) and PKN1 cell lysate. Samples were incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of PKN1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of PKN1 in Hela cells (green). Sample was incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PKN1 in paraffin-embedded colon cancer tissue. Sample was incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:200-1:1000 followed by DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of PKN1 in HeLa cells (green). Sample was incubated with PKN1 monoclonal antibody (Product # MA5-31849) using a dilution of 1:200-1:400 followed by negative control (red).
