MA1-06503

Hisatsune A, Nakayama H, Kawasaki M, Horie I, Miyata T, Isohama Y, Kim KC, Katsuki H
Biochemical and biophysical research communications 2011 Feb 18;405(3):377-81

Human embryonic stem cell-derived keratinocytes exhibit an epidermal transcription program and undergo epithelial morphogenesis in engineered tissue constructs.

Metallo CM, Azarin SM, Moses LE, Ji L, de Pablo JJ, Palecek SP
Tissue engineering. Part A 2010 Jan;16(1):213-23

Secondary involvement of the breast in T-cell non-Hodgkin lymphoma, an unusual example mimicking inflammatory breast carcinoma.

Kelten C, Kabukcu S, Sen N, Teke Z, Yaren A, Erdem E, Duzcan E
Archives of gynecology and obstetrics 2009 Jul;280(1):149-52

Myointimoma of the glans penis.

Vardar E, Gunlusoy B, Arslan M, Kececi S
Pathology international 2007 Mar;57(3):158-61

Pulmonary large cell carcinoma with rhabdoid phenotype.

Yilmazbayhan D, Ates LE, Dilege S, Gulluoglu M, Tanju S, Kalayci G
Annals of diagnostic pathology 2005 Aug;9(4):223-6

Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells.

Stanley CM, Phillips TE
The American journal of physiology 1999 Jul;277(1):G191-200

Selective secretion and replenishment of discrete mucin glycoforms from intestinal goblet cells.

Stanley CM, Phillips TE
The American journal of physiology 1999 Jul;277(1 Pt 1):G191-200

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MUC1 Monoclonal Antibody (GP1.4)(Product # MA1-06503) and a ~180, 260 and 460kDa band corresponding to MUC1 was observed across T47D, MCF-7 and DU-145 compared to HCT-116 and IMR-32. Membrane enriched extracts (50 µg lysate) of T-47D (Lane 1), MCF7 (Lane 2), DU 145 (Lane 3), HCT 116 (Lane 4), IMR-32 (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556). A streak like pattern was observed in the positive cell lines

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of MUC1 was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MUC1 Monoclonal Antibody (GP1.4) (Product # MA1-06503) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), at 1:2000 dilution, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Plasma membrane and cytoplasm localization. Panel e represents HCT116. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Knockdown of MUC1 suppresses tumor growth and JNK/pSmad3L/c-Myc pathway in mice (A) BALB/c nude mice subcutaneous transplant tumor model was established using SMMC-7721, NC, MR1-D4 and MR1-D9 cells. (B) Tumors in mice were dissected and photographed on day 21 post-injection. The images were captured showing tumor sizes for each group. (C) Tumors from mice were detected for the expression of MUC1, p-JNK, p-Smad3L (Ser-213) and c-Myc by immunohistochemical staining. Sections were examined on an inverted fluorescence microscope (IX71; Olympus) at 100 x magnification. The scale bar indicates 50 mum.