14-9893-80
antibody from Invitrogen Antibodies
Targeting: MUC1
ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM
Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 14-9893-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD227 (Mucin 1) Monoclonal Antibody (SM3), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer.
- Antibody clone number
- SM3
- Concentration
- 0.5 mg/mL
Submitted references Patterns of MUC1 tissue expression defined by an anti-MUC1 cytoplasmic tail monoclonal antibody in breast cancer.
Immunohistochemical study of the expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues.
Mice with spontaneous pancreatic cancer naturally develop MUC-1-specific CTLs that eradicate tumors when adoptively transferred.
Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells.
Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides.
A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.
Croce MV, Isla-Larrain MT, Rua CE, Rabassa ME, Gendler SJ, Segal-Eiras A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Jun;51(6):781-8
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 Jun;51(6):781-8
Immunohistochemical study of the expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues.
Pereira MB, Dias AJ, Reis CA, Schmitt FC
Journal of clinical pathology 2001 Mar;54(3):210-3
Journal of clinical pathology 2001 Mar;54(3):210-3
Mice with spontaneous pancreatic cancer naturally develop MUC-1-specific CTLs that eradicate tumors when adoptively transferred.
Mukherjee P, Ginardi AR, Madsen CS, Sterner CJ, Adriance MC, Tevethia MJ, Gendler SJ
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 15;165(6):3451-60
Journal of immunology (Baltimore, Md. : 1950) 2000 Sep 15;165(6):3451-60
Comparison of O-linked carbohydrate chains in MUC-1 mucin from normal breast epithelial cell lines and breast carcinoma cell lines. Demonstration of simpler and fewer glycan chains in tumor cells.
Lloyd KO, Burchell J, Kudryashov V, Yin BW, Taylor-Papadimitriou J
The Journal of biological chemistry 1996 Dec 27;271(52):33325-34
The Journal of biological chemistry 1996 Dec 27;271(52):33325-34
Cytotoxic T cells from ovarian malignant tumors can recognize polymorphic epithelial mucin core peptides.
Ioannides CG, Fisk B, Jerome KR, Irimura T, Wharton JT, Finn OJ
Journal of immunology (Baltimore, Md. : 1950) 1993 Oct 1;151(7):3693-703
Journal of immunology (Baltimore, Md. : 1950) 1993 Oct 1;151(7):3693-703
A short sequence, within the amino acid tandem repeat of a cancer-associated mucin, contains immunodominant epitopes.
Burchell J, Taylor-Papadimitriou J, Boshell M, Gendler S, Duhig T
International journal of cancer 1989 Oct 15;44(4):691-6
International journal of cancer 1989 Oct 15;44(4):691-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human infiltrating ductal carcinoma, using 5 µg/mL of Mouse IgG1 Isotype Control Purified (Product # 14-4714-82) (left) or Anti-Human CD227 (Mucin 1) Purified (right) followed by Anti-Mouse Biotin, and DAB visualization.Nuclei are counterstained with hematoxylin.