42-9893-80
antibody from Invitrogen Antibodies
Targeting: MUC1
ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- 42-9893-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD227 (Mucin 1) Monoclonal Antibody (SM3), eFluor™ 615, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SM3 monoclonal antibody reacts with the under-glycosylated form of human Mucin 1 (MUC1, CD227), a large glycoprotein belonging to the mucin protein family. Mucin 1 contains a polypeptide core consisting of multiple tandem repeats that become highly glycosylated. Mucin 1 is typically expressed in ductal or glandular epithelial cells and is localized to the apical membrane. In cancerous cells, Mucin 1 expression is increased and membrane-specific localization is lost resulting in expression throughout the membrane and cytoplasm. High levels of under-glycosylated Mucin 1 are thought to affect cell behavior during both invasion and metastasis as well as in immune recognition. In addition, under-glycosylated Mucin 1 is shed from the epithelial cell surface and can be detected in circulation. Alterations in Mucin 1 glycosylation are found in most adenocarcinomas of the breast, lung, pancreas, prostate, and ovary. Mucin 1 has recently been shown to co-localize and interact with members of the erbB receptor kinase family, proteins that are upregulated in more aggressive forms of breast cancer. Please note this antibody sees a distinct epitope from other Mucin 1 antibodies. Applications Reported: This SM3 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections (IHC-P) and immunocytochemistry (ICC). Applications Tested: This SM3 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma tissue and by immunocytochemistry on fixed MCF7 cells at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SM3
- Vial size
- 25 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Lipid-polymer nanoparticles encapsulating curcumin for modulating the vascular deposition of breast cancer cells.
Palange AL, Di Mascolo D, Carallo C, Gnasso A, Decuzzi P
Nanomedicine : nanotechnology, biology, and medicine 2014 Jul;10(5):991-1002
Nanomedicine : nanotechnology, biology, and medicine 2014 Jul;10(5):991-1002
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on formalin-fixed paraffin embedded human breast carcinoma using 20 µg/mL of Mouse IgG1 Isotype Control eFluor® 615 (left) or 20 µg/mL Anti-Human CD277 (Mucin 1) eFluor® 615 (right). Nuclei are counterstained with DAPI.