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LearnMA5-15131
antibody from Invitrogen Antibodies
Targeting: MUC1
ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-15131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MUC1 Monoclonal Antibody (S.854.6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other mucin proteins.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S.854.6
- Vial size
- 100 µL
- Concentration
- 635 µg/mL
- Storage
- -20°C
Submitted references Radiotherapy and MVA-MUC1-IL-2 vaccine act synergistically for inducing specific immunity to MUC-1 tumor antigen.
Hillman GG, Reich LA, Rothstein SE, Abernathy LM, Fountain MD, Hankerd K, Yunker CK, Rakowski JT, Quemeneur E, Slos P
Journal for immunotherapy of cancer 2017;5:4
Journal for immunotherapy of cancer 2017;5:4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MUC1 in extracts from PL45 and MCF-7 cells using MUC1 monoclonal antibody (Product # MA5-15131).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MUC1 Monoclonal Antibody (S.854.6)(Product # MA5-15131) and a ~160kDa band corresponding to MUC1 was observed across T47D and DU-145 in comparison to MCF-7, HCT-116 and IMR-32. Membrane enriched extracts (30 µg lysate) of T-47D (Lane 1), MCF7 (Lane 2), DU 145 (Lane 3), HCT 116 (Lane 4), IMR-32 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).^^18
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MUC1 in paraffin-embedded human colon carcinoma using a MUC1 monoclonal antibody (Product # MA5-15131).
