- MA5-14077 - Invitrogen Antibodies MUC1 antibody

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Huisman BW, Cankat M, Bosse T, Vahrmeijer AL, Rissmann R, Burggraaf J, Sier CFM, van Poelgeest MIE
Cancers 2021 Nov 29;13(23)

Oncocytic papillary renal cell carcinoma: A clinicopathological and genetic analysis and indolent clinical course in 14 cases.

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Pathology, research and practice 2017 Jan;213(1):1-6

Primary acinic cell carcinoma of the breast: a case report and review of the literature.

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Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.

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Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.

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An immunohistochemical study of the origin of the solid strand in syringoma, using carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 5.

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Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Yin WH, Li J, Chan JK
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Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.

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Guyotat J, Metellus P, Giorgi R, Barrie M, Jouvet A, Fevre-Montange M, Chinot O, Durand A, Figarella-Branger D
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An immunohistochemical profile of giant cell carcinoma of the larynx.

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Anti-tumor effect of the anti-KL-6/MUC1 monoclonal antibody through exposure of surface molecules by MUC1 capping.

Doi M, Yokoyama A, Kondo K, Ohnishi H, Ishikawa N, Hattori N, Kohno N
Cancer science 2006 May;97(5):420-9

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Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia 2005 Sep;12(7):829-32

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Collagenous fibroma (desmoplastic fibroblastoma).

Dagli M, Eryilmaz A, Acar A, Kulacoglu S, Akmansu H
Yonsei medical journal 2004 Oct 31;45(5):941-3

Prognostic significance of mucin expression in gastric carcinoma.

Kocer B, Soran A, Kiyak G, Erdogan S, Eroglu A, Bozkurt B, Solak C, Cengiz O
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Prognostic significance of mucin expression in gastric carcinoma.

Kocer B, Soran A, Kiyak G, Erdogan S, Eroglu A, Bozkurt B, Solak C, Cengiz O
Digestive diseases and sciences 2004 Jun;49(6):954-64

A confirmatory report for the close interaction of Helicobacter pylori with gastric epithelial MUC5AC expression.

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A confirmatory report for the close interaction of Helicobacter pylori with gastric epithelial MUC5AC expression.

Kocer B, Ulas M, Ustundag Y, Erdogan S, Karabeyoglu M, Yldrm O, Unal B, Cengiz O, Soran A
Journal of clinical gastroenterology 2004 Jul;38(6):496-502

Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands.

Liu B, Lague JR, Nunes DP, Toselli P, Oppenheim FG, Soares RV, Troxler RF, Offner GD
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2002 Jun;50(6):811-20

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-MUC1 Monoclonal Antibody (E29) (Product # MA5-14077) and a 160 kDa band corresponding to MUC1 was observed across tissues tested. Whole cell extracts (30 µg lysate) of Mouse Stomach (Lane 1), Mouse Liver (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Expression of MUC1 was observed higher in mouse stomach as compared to mouse liver.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of MUC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MUC1 Monoclonal Antibody (LH39) (Product # MA5-14077) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ 647 Phalloidin (Product #A22287, 1:300 dilution). Panel d represents the merged image showing cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody using peroxidase-conjugate and AEC chromogen. Note membrane and cytoplasmic staining of tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Mucin 1/MUC-1 (E29) showing staining in the cytoplasm and membrane of paraffin-embedded human uterus tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 1/MUC-1 Antibody (E29) Mouse Monoclonal Antibody (Product # MA5-14077) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Mucin 1/MUC-1 (E29) showing staining in the cytoplasm and membrane of paraffin-embedded mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 1/MUC-1 Antibody (E29) Mouse Monoclonal Antibody (Product # MA5-14077) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Mucin 1/MUC-1 (E29) showing staining in the membrane and weak cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Mucin 1/MUC-1 Antibody (E29) Mouse Monoclonal Antibody (Product # MA5-14077) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

MA5-14077

Antibody data