HPA008855

antibody from Atlas Antibodies

Targeting: MUC1 ADMCKD, ADMCKD1, CD227, MCD, MCKD, MCKD1, PEM, PUM

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA008855

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008855, RRID:AB_1846283

Product name

Anti-MUC1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AVCQCRRKNYGQLDIFPARDTYHPMSEYPTYHTHG
RYVPPSSTDRSPYEKVSAGNGGSSLSYTNPAVAAT
SANL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Single-cell analysis reveals a stem-cell program in human metastatic breast cancer cells.

Lawson DA, Bhakta NR, Kessenbrock K, Prummel KD, Yu Y, Takai K, Zhou A, Eyob H, Balakrishnan S, Wang CY, Yaswen P, Goga A, Werb Z
Nature 2015 Oct 1;526(7571):131-5

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human stomach and liver tissues using Anti-MUC1 antibody. Corresponding MUC1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows strong luminal membranous positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN