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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21489 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PGCP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji. Predicted reactivity: Mouse (90%), Rat (88%), Pig (90%), Bovine (88%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.68 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PGCP using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PGCP polyclonal antibody (Product # PA5-21489) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PGCP Polyclonal Antibody (Product # PA5-21489). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PGCP Polyclonal Antibody (Product # PA5-21489) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PGCP Polyclonal Antibody detects PGCP protein at cytosol on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. PGCP Polyclonal Antibody (Product # PA5-21489) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
