Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP14501PU-N - Provider product page
- Provider
- OriGene
- Product name
- Pumilio 2 (PUM2) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Pumilio 2 (PUM2) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PUM2
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 1. Western blot analysis of PUM2 (arrow) using PUM2 Antibody . 293 cell lysates (2 ug/lane) either non-transfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2)(Origene Technologies).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 2. Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with PUM2 Antibody (S182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure 3. Flow cytometric analysis of HepG2 cells using PUM2 Antibody (S182)(Bottom Histogram) compared to a Negative Control cell (Top Histogram). FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC