GTX22743
antibody from GeneTex
Targeting: THRA
AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX22743 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22743, RRID:AB_374338
- Product name
- Thyroid Hormone Receptor antibody [C3]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Simian, Xenopus
- Host
- Mouse
- Storage
- -20¢X C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot of human thyroid hormone receptor.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Thyroid Hormone Receptor in HeLa Cells. Cells were grown on slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with Thyroid Hormone Receptor antibody [C3] at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a proper secondary antibody. Thyroid Hormone Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal biopsies of deparaffinized human thyroid tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed at a dilution of 1:20 with or without Thyroid Hormone Receptor antibody [C3] overnight at 4¢XC in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.