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LearnAP09375PU-S
antibody from OriGene
Targeting: THRA
AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09375PU-S - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-THRA Antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-THRA Antibody, Aff - Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25ul
- Concentration
- 0.89 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
- Validation comment
- WB
