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LearnMA1-4674
antibody from Invitrogen Antibodies
Targeting: THRA
AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- MA1-4674 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- THRA Monoclonal Antibody (2103)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine, mouse and non-human primate based on 100% sequence homology. This antibody is specific for the ~50 kDa human THR-alpha1 and the ~58 kDa human THR-alpha2 proteins.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2103
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Human parvovirus B19 infection leads to downregulation of thyroid, estrogen, and retinoid hormone receptor expression.
Ignatovich IV, Hobbs JA
Virology 2013 Nov;446(1-2):173-9
Virology 2013 Nov;446(1-2):173-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of brain lysate showing specific immunolabeling of the ~50 kDa THR-alpha1 (bottom) and the ~58 kDa THR-alpha2 (top) proteins.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of THRA in rat hippocampal lysate showing specific immunolabeling for a band at ~50 kDa corresponding to TR-alpha 1 and a ~58 kDa band corresponding to TR-alpha 2 receptors for THRA monoclonal antibody (Product # MA1-4674).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of THRA was performed using 70 confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with THRA Monoclonal Antibody (2103) (Product # MA1-4674) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
