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LearnAP09375PU-N
antibody from Acris Antibodies GmbH
Targeting: THRA
AR7, EAR-7.1/EAR-7.2, ERBA, ERBA1, NR1A1, THRA1, THRA2, THRA3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09375PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09375PU-N, RRID:AB_2036023
- Product name
- anti THRA / ERBA1
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a region near the amino terminus of Human THRA isoform 1 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.89 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA.
