MA3-069

antibody from Invitrogen Antibodies

Targeting: TAF10 TAF2A, TAF2H, TAFII30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-069

Provider

Invitrogen Antibodies

Product name

TAF10 Monoclonal Antibody (23TA-1H8)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA3-069 detects TAF10 from human, rat, and non-human primate samples. MA3-069 does not detect the TAF10 in mouse samples MA3-069 has been successfully used in Western blot, immunofluorescence and flow cytometry applications. The MA3-069 immunogen is the synthetic peptide coupled to ovalalbumin aa 1 to aa 20.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

23TA-1H8

Vial size

50 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TAF10 was performed by loading 20 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TAF10 was detected at 30 kDa using a TAF10 mouse monoclonal antibody (Product # MA3-069) at a dilution of 1:1000 in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IImmunofluorescent analysis of TAF10 in HepG2 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a TAF10 mouse monoclonal antibody (Product # MA3-069) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for at least 30 minutes at room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IImmunofluorescent analysis of TAF10 in HepG2 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a TAF10 mouse monoclonal antibody (Product # MA3-069) at a dilution of 1:2000 in blocking buffer for 1 hour at room temperature, and then incubated with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:1000 for at least 30 minutes at room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of TAF10 was done on HeLa cells. Cells were fixed, permeabilized and stained with a TAF10 mouse monoclonal antibody (Product # MA3-069, red histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650 conjugate (Product # 84545) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells and the blue histogram represents no-primary-antibody control.