702277

antibody from Invitrogen Antibodies

Targeting: TNFRSF25 APO-3, DDR3, DR3, LARD, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR

Western blot

Antibody data

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Validation data

Product number: 702277 - Provider product page
Provider: Invitrogen Antibodies
Product name: DR3 Recombinant Rabbit Monoclonal Antibody (11H6L9)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: 11H6L9
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TNFRSF25 protein structure - 702277 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis was performed on fixed and permeabilized K-562 cells for detection of endogenous DR3/TNFRSF25 using Anti-DR3/TNFRSF25 Recombinant Rabbit Monoclonal Antibody (Product # 702277, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DR3/TNFRSF25 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of DR3/TNFRSF25. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis was performed on fixed and permeabilized Ramos cells for detection of endogenous DR3/TNFRSF25 using Anti-DR3/TNFRSF25 Recombinant Rabbit Monoclonal Antibody (Product # 702277, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DR3/TNFRSF25 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating localization of DR3/TNFRSF25 in the membrane. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.