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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-31352 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HRSP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: HCT116.
- Concentration
- 1 mg/mL
Submitted references Endoribonucleolytic Cleavage of m(6)A-Containing RNAs by RNase P/MRP Complex.
Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay.
Park OH, Ha H, Lee Y, Boo SH, Kwon DH, Song HK, Kim YK
Molecular cell 2019 May 2;74(3):494-507.e8
Molecular cell 2019 May 2;74(3):494-507.e8
Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay.
Park OH, Park J, Yu M, An HT, Ko J, Kim YK
Genes & development 2016 Sep 15;30(18):2093-2105
Genes & development 2016 Sep 15;30(18):2093-2105
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HRSP12 Polyclonal Antibody (Product # PA5-31352). Sample (30 µg of whole cell lysate). Lane A: HCT116. 15% SDS PAGE. HRSP12 Polyclonal Antibody (Product # PA5-31352) diluted at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HRSP12 Polyclonal Antibody detects HRSP12 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: HRSP12 protein stained by HRSP12 Polyclonal Antibody (Product # PA5-31352) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HRSP12 Polyclonal Antibody detects HRSP12 protein at nucleus on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. HRSP12 Polyclonal Antibody (Product # PA5-31352) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
