AF2526

antibody from Novus Biologicals

Targeting: IL27 IL-27, IL-27A, IL27A, IL27p28, IL30, MGC71873, p28

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2526

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-27 Antibody

Antibody type

Polyclonal

References

Submitted references

Type I IFN augments IL-27-dependent TRIM25 expression to inhibit HBV replication.

Tan G, Xiao Q, Song H, Ma F, Xu F, Peng D, Li N, Wang X, Niu J, Gao P, Qin FX, Cheng G
Cellular & molecular immunology 2018 Mar;15(3):272-281

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Interleukin 27 is increased in carotid atherosclerosis and promotes NLRP3 inflammasome activation.

Gregersen I, Sandanger Ø, Askevold ET, Sagen EL, Yang K, Holm S, Pedersen TM, Skjelland M, Krohg-Sørensen K, Hansen TV, Dahl TB, Otterdal K, Espevik T, Aukrust P, Yndestad A, Halvorsen B
PloS one 2017;12(11):e0188387

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HIV and HIV-Tat inhibit LPS-induced IL-27 production in human macrophages by distinct intracellular signaling pathways.

Gajanayaka N, O'Hara S, Konarski Y, Fernandes J, Muthumani K, Kozlowski M, Angel JB, Kumar A
Journal of leukocyte biology 2017 Sep;102(3):925-939

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RIG-I-like receptor activation by dengue virus drives follicular T helper cell formation and antibody production.

Sprokholt JK, Kaptein TM, van Hamme JL, Overmars RJ, Gringhuis SI, Geijtenbeek TBH
PLoS pathogens 2017 Nov;13(11):e1006738

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Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4.

Garg A, Trout R, Spector SA
Scientific reports 2017 Mar 24;7:44485

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IL-27 and type 2 immunity in asthmatic patients: association with severity, CXCL9, and signal transducer and activator of transcription signaling.

Xie M, Mustovich AT, Jiang Y, Trudeau JB, Ray A, Ray P, Hu H, Holguin F, Freeman B, Wenzel SE
The Journal of allergy and clinical immunology 2015 Feb;135(2):386-94

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Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.

Gringhuis SI, Kaptein TM, Wevers BA, van der Vlist M, Klaver EJ, van Die I, Vriend LE, de Jong MA, Geijtenbeek TB
Nature communications 2014 Oct 3;5:5074

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Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis.

Fitzgerald DC, Fonseca-Kelly Z, Cullimore ML, Safabakhsh P, Saris CJ, Zhang GX, Rostami A
Journal of immunology (Baltimore, Md. : 1950) 2013 Apr 1;190(7):3225-34

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B cells as a therapeutic target for IFN-β in relapsing-remitting multiple sclerosis.

Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4518-26

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Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.

Kalliolias GD, Gordon RA, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-27 by Western Blot. Western blot shows lysates of MT-2 human T cell line and CHO Chinese hamster ovary cell line either mock transfected or transfected with human IL-27 p28/IL-27A. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for the IL-27 EBI3/IL-27B at approximately 32 kDa and IL-27 p28/IL-27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IL-27 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IL-27 Antibody. Recombinant Human IL-27 (Catalog # 2526-IL) reduces the Encephalo-myocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-27 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-27 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2526). The ND₅₀ is typically 1-5 µg/mL.